The ultimate brain fixation method–focused microwave energy
The NEW method for reliable and rapid fixation of brain tissue
If doing protein phosphorylation — no other method compares! Microwave fixation is the fastest and most effective known method of halting brain chemical activity. Several important brain chemicals, including acetylcholine, dopamine, DARRP, HVA, and c-AMP begin changing state rapidly at death.
The 10 to 90 seconds required for complete freezing following decapitation into liquid nitrogen is sufficient for significant degradation, phosphorylation, or other changes to occur.
Microwave fixation, however, yields chemical levels like those in the living brain. Levels of enzyme-regulated neurotransmitters, and metabolic substances in the brain, are stopped at the levels that existed in the living animal, while brain size and structure remain virtually unchanged.
Patented Microwave Focus Applicators
Microwaves are channeled and focused by the wave guide which focuses the microwaves from above the head, rather than in front. The entire rat head is placed in a uniform microwave field. Movements of the head do not change the field strength or microwave distribution. Thus, uniform fixation of the whole brain is achieved, without “over cooking” and damage to the hypothalamus.
Muromachi’s unique applicators provide protection to the researcher and also compensate for individual differences between animals, giving more reproducible results.
Compact Mobile Cabinet
The Muromachi Microwave Fixation System is enclosed in one cabinet, mounted on 5" casters, with a single convenient control panel. Holders are available for animals from 20 to 500 gms. Order appropriate applicators and holders for your application.
Eliminates the use of other fixation methods such as paraformaldehyde/formalin